T4 DNA Ligase is highly efficient, accurate and rapid enzyme designed for an efficient ligation of cohesive and blunt ended DNA insert into plasmid vectors in 5-15 minutes. It is based on the combination of T4 DNA Ligase with a Premium 5x Ligation Buffer. The ligation reaction mixture is used directly for bacterial transformation using conventional transformation procedures. It enables sticky end or blunt end DNA ligation in only 5 minutes at Room Temperature.
Recombinant: isolated from E. coli.
Highly efficient: 3,500 white colonies and 10 blue colonies expected under optimal conditions.
Fast and easy protocol: get 24 % of efficiency at 5 minutes and 75 % at 15 minutes.
Powerful: allows cloning insert amounts < 1 ng/kb.
Versatile: enables sticky end or blunt end DNA Ligation.
Risk-free: product covered by our Quality 100% Guarantee.