The Pierce™ Chemiluminescent Nucleic Acid Detection Module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.
Fast and sensitive
Versatile kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
Kit contains only the critical detection components for those who already have biotin-labeled probes and an optimised hybridisation method developed
The system uses an enhanced luminol substrate for horseradish peroxidase (HRP) with optimised blocking and wash steps that together produce sensitivity equivalent to radioactive (32P) systems and provide a clean, consistent background required for publication-quality data.
Delivery information: Kit contains stabilised Streptavidin-HRP conjugate, 1.5 ml; chemiluminescent substrate (luminol/enhancer solution, 80 ml and stable peroxide solution, 80 ml); blocking buffer, 500 ml; 4X wash buffer, 500ml; and substrate equilibration buffer, 500ml.
Sufficient for 800 cm2 of membrane (10 mini-gel blots)
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