Description: Maxima® SYBR Green qPCR Master Mix (2X), 10×1.25 ml (for 1000×25 µl reactions), with ROX (250 µl) and 10×1.25 ml nuclease-free water
The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
Specificity – eliminates non specific amplification and formation of primer dimers
Sensitivity – detects low copy number targets
ROX solution provided in a separate tube
Wide linear range – accurate quantification across 9 orders of magnitude
Can be used on most real time PCR instruments
Ready to use
Delivery information: Supplied with 50 µM ROX solution and nuclease-free water.
For more details, please follow https://uk.vwr.com/store/product/9081787/maximatm-sybr-green-qpcr-master-mix-2x-with-rox
