T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
For covalently linking DNA fragments in as little as 30 minutes
Single dose, ambient-temperature-stable reaction mixes
Delivery information: Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 µl, yields a solution of reaction buffer, T4 DNA ligase, and ATP.
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