Synonyms: 3',6'-bis(acetyloxy)-5-(chloromethyl)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one , 5-Chloromethylfluorescein diacetate
Green CMFDA is a cell-permeable fluorescent probe that freely enters living cells, where it is transformed into a cell-impermeable, soluble, fluorescent compound. The resulting molecule does not affect cell viability or proliferation and stably fluoresces for at least 72 hours, allowing for its use in cell tracking. It is also transferred to daughter cells.
Emission 517 nm
Excitation 492 nm
Formulation A crystalline solid
λmax 220, 291 nm
Green CMFDA is brightly fluorescent with standard filters (ex/em max = 492/517 nm) and is often used with additional fluorescent dyes and proteins.
Formula: C₂₅H₁₇ClO₇
Storage Temperature: Freezer
CAS Number: 136832-63-8
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