pSpark® I DNA Cloning Kit

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes.
Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
High stability: eliminates cloning bias or pitfalls.
Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert.
Compatible with blue/white screening.
Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
Eliminates positive selection vector.
High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
Risk-free: product covered by our Quality 100% Guarantee.

For More Details:
https://lifescience.canvaxbiotech.com/product/pspark-i-dna-cloning-kit/

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MOHAMED MOHAMED

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19-05-2021 09:22:48

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