pSpark® TA DNA Cloning Kit

Manufacturer: Canvax
Availability: in stock 0 item(s)
Product Code: P21741654
SKU: C0020
UOM: 20 rxn
Product Sub Group: Cloning system

pSpark® TA is efficient, stable and easy-to-use DNA cloning vector based on an optimized TA technology for cloning single 3 ́-adenine overhanging DNA. The vectors are prepared by digestion of pSpark® TA at EcoRV site and the subsequent addition of a single thymidine at each 3 ́- end to allow cloning Taq DNA Polymerase amplified DNA fragments. Its exclusive procedure offers greater efficiency and less background of blue colonies than the others TA vectors.

  • Efficient: more than 600 white positive colonies expected under optimal conditions.
  • Easy-to-use: eliminates screening of recombinants due to its low background (4%).
  • High stability: vector without cloning bias due to transcription of toxic genes.
  • Fast protocol: ligation time from 60 minutes to overnight.
  • Compatible: with direct cloning of PCR products.
  • Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase.
  • Cost avoidance: avoids primer phosphorylation.
  • Risk-free: product covered by our Quality 100% Guarantee.
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