X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) is a chromogenic substrate for beta-galactosidase.
β-Galactosidase cleaves the substrate and releases colorless galactose and 5-Bromo-4-chloro-3-indoxyl (X). The indoxyl will be oxidized to the insoluble 5,5′-dibromo-4,4′-dichloro-indigo, forming an intense blue precipitate.
Purity: > 99% confirmed by HPLC.
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