pSpark® TA Done is efficient, stable and easy-to-use DNA cloning vector based on an improved TA technology that offers all of the advantages of pSpark® TA with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.
Convenient: recognition sites for EcoRI and NotI flanking the insertion site.
Flexible: allows removing the desired insert DNA with other restriction digestion.
Efficient: more than 600 white positive colonies expected under optimal conditions.
Stable: without cloning bias due to transcription of toxic genes.
Easy-to-use: eliminates screening of recombinants due to its low background (4%).
Fast protocol: ligation time from 60 minutes to overnight.
Compatible: with direct cloning of PCR products.
Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase.
Cost avoidance: avoids primer phosphorylation.
Risk-free: product covered by our Quality 100% Guarantee.
For further details:https://lifescience.canvaxbiotech.com/product/pspark-ta-done-dna-cloning-kit/