One-Step RT PCR Kit is designed for a Highly Efficient cDNA Synthesis and PCR in a single tube. The kit includes a PCR MasterMix supplied in a 2x concentration to perform standard PCR.
MasterMix contains all the reagents needed for running PCR reactions (except PCR primers and template). In addition, a separate RT Mix composed by a balanced mixture of both RTase and RNase Inhibitor is also provided.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA Polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA Polymerase exponentially amplifies the double-stranded DNA template.