DNA Polymerase I is a multifunctional enzyme that combines a DNA polymerase activity, a 5´→ 3´exonuclease activity and a 3’→5′ proofreading exonuclease activity. The 5´→3´ exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting points for labelling DNA by nick translation.
Native: isolated from E. coli cells with a cloned fragment of the polA gene.
DNase I-dependent nick translation: second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs.
Complete solution: supplied with 10x Reaction Buffer.
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