Klenow Fragment is the Large Fragment of DNA Polymerase I. It shows 5’→3′ polymerase activity and 3’→5′ exonuclease (proofreading) activity, but lacks 5’
Recombinant: isolated from E. coli cells with a cloned fragment of the polA gene.
DNase I-dependent nick translation: second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs.
Generates probes using random primers.
Creates blunt ends.
Complete solution: supplied with 10x Reaction Buffer.
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