On-membrane DNase I Set (RNase-Free) is designed for a highly efficient on-column digestion of DNA during critical RNA purification procedures for downstream applications requiring DNA-free total RNA.
DNase I, RNase free, recombinant, is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri-, and oligonucleotide products (on average producing tetranucleotides) with 5′-phosphorylated and 3′-hydroxylated ends. DNAse I acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids, and chromatin. DNase I requires bivalent cations (Mg2+ and Ca2+) for maximal activity.
Highly Efficient on-column digestion of DNA thanks to its innovative OM Buffer composition that ensures that the RNA remains bound to the column.
RNAse-free.
Recombinant Bovine Pancreatic DNAse I, purified from coli (29 kDa monomer).
Optimized Buffer included for on-column DNase digestion.
