On-membrane DNase I Set (RNase-Free) is designed for a highly efficient on-column digestion of DNA during critical RNA purification procedures for downstream applications requiring DNA-free total RNA.
DNase I, RNase free, recombinant, is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri-, and oligonucleotide products (on average producing tetranucleotides) with 5′-phosphorylated and 3′-hydroxylated ends. DNAse I acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids, and chromatin. DNase I requires bivalent cations (Mg2+ and Ca2+) for maximal activity.
Highly Efficient on-column digestion of DNA thanks to its innovative OM Buffer composition that ensures that the RNA remains bound to the column.
Recombinant Bovine Pancreatic DNAse I, purified from coli (29 kDa monomer).
Optimized Buffer included for on-column DNase digestion.