Tris-Glycine Buffer (TG) is a high quality and convenient running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins.
Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.
It is also used to make Tris-glycine/20% methanol Western transfer buffer, which is the most frequently used protein transfer buffer for wet blot transfers.
Pouch format
10X solution
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