Non-viral Mammalian Expression Kit
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ I (with Puromycin resistance) incorporates the cytomegalovirus early promoter that precedes 2A sequence in frame with truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It could be visualized by cytometry using specific antibody labelled with FITC or similar and also, it could be enriched from negative clones with magnetic beads bearing anti-ΔNGFR antibody.
For more details, please follow https://lifescience.canvaxbiotech.com/product/ponebyone-i-puro-mammalian-expression/
