Non-viral Mammalian Expression Kit
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ III (with Puromycin resistance) incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence, in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It can be visualized by cytometry using a specific antibody labelled with FITC or a similar method, and also, it can be enriched from negative clones with magnetic beads bearing an anti-ΔNGFR antibody.
For more details, please follow https://lifescience.canvaxbiotech.com/product/ponebyone-iii-puro-mammalian-expression/
