Universal Non-Viral Mammalian Expression Kit
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology.
pOnebyOne™ FREE-2 Bicistronic Mammalian Expression Kit allows co-expression of two genes of interest driven by human cytomegalovirus early promoter. It is based in 2A sequence from Thosea Asigna virus which is flanked by two multiple cloning sites. T2A sequence leads to the highest level of protein expression at the second gene position compared to
other 2As (Liu et al, 2017).
The mechanism of 2A self-cleavage is a ribosome skipping process where the ribosome skips the formation of a glycil-prolyl peptide bond at the C-terminus of 2A.
Stable mammalian cells could be selected by Neomycin Resistance.
For more details,please follow https://lifescience.canvaxbiotech.com/product/ponebyone-free-2-bicistronic-mammalian-expression-kit/
