FDG β-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Lac Z is often used reporter gene in experiments transfection because the β-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. β-galactosidase performs the hydrolysis of fluorescein di-β-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of β-galactosidase is proportional to fluorescence produced.measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
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