A novel Coronavirus, currently termed COVID-19 (2019-nCoV or SARS-CoV-2), was announced as the etiological agent of cases of ongoing Pneumonia outbreak in Wuhan City (China). This kit describes the use of real time RT-PCR for the in vitro detection of SARS-CoV-2 in respiratory specimens (sputum; nasopharyngeal, oropharyngeal aspirates, washes or swabs; tracheal aspirates).
qMAXSen™ Coronavirus (SARS-CoV-2) RT-qPCR Detection Kit [N and RNAse P genes-based Assay] allows efficient cDNA synthesis and Real-Time PCR in a single tube. The kit includes a RT-qPCR MasterMix supplied in a 4x and a vial with Ribonuclease Inhibitor.
Also, the Kit contains two sets of Primers and Fluorescent Probe designed according to CDC (Centers for Disease Control and Prevention) to target regions of the Virus Nucleocapsid (N) Gene, and to detect the human RNase P used as a control to assess specimen quality. The probes are readout in different channels. Coronavirus SARS-CoV-2 RNA target is
amplified and detected in the FAM channel, Human RNase P target is amplified and detected in the HEX, VIC or JOE channel (depending on the equipment used).
For more details, please follow https://lifescience.canvaxbiotech.com/product/ncov-cdc-assays/