The E.Z.N.A.® Tissue DNA Kit offers a versatile and cost-effective method for the isolation of DNA from a wide variety of samples including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc. The DNA purification process is simplified with HiBind® Mini Column technology into four quick “lyse-bind-wash-elute” steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation, use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion.
Rapid – DNA isolation in less than 20 minutes post-lysis
Versatile – Single kit for multiple sample types
Specialized buffer system – Optimized buffers for higher yields
Safe – No phenol/chloroform extractions
High-quality – DNA is suitable for a variety of downstream applications
Downstream Applications: PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion
Starting Material: Tissues, cultured cells, mouse tail snips, paraffin-embedded tissues, whole blood, body fluids, buccal swabs
Starting Amount: 30 mg, or 5 x 106 cultured cells
Elution Volume: 100-200 μL
DNA Binding Technology: Silica mini spin column
Throughput: 1-24
Processing Mode: < 20 min (post-lysis)
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