The E.Z.N.A.® DNA/RNA Isolation Kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenized in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind® RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, the maximum yield of DNA and RNA can be purified from the entire sample.
Downstream Application: PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume: 40-70 µL for RNA; 50-100 µL for DNA
Starting Material: Cultured cells and animal tissues
Starting Amount: Up to 1X107 cells; up to 30 mg tissue
RNA Yield: RNA in one fraction; DNA in another
Processing Mode: Manual, centrifugation
Throughput: 1 - 24
RNA Binding Technology: Silica Mini spin column
Binding Capacity: 100 µg for DNA and RNA
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