The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNase’s and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.
Downstream Application: PCR, qPCR, real-time RT-PCR, micro-array, Northern blot, poly-A purification
Elution Volume: 15-30 μL
Starting Material: FFPE tissue
Starting Amount: 3-4 sections with a thickness of 10 μm
RNA Yield: Up to 50 µg
Processing Mode: Manual, centrifugation
Throughput: 1 - 24
RNA Binding Technology: Silica Micro spin column
Binding Capacity: 50 µg
For more details, please follow https://uk.vwr.com/store/product?keyword=R6954-00
