The E.Z.N.A.® Cycle-Pure Kit is designed for the rapid purification of single or double-stranded DNA from PCR and other enzymatic reactions. The system follows a “bind-wash-elute” procedure and completely removes primers, nucleotides enzymes, salts, and other impurities from a DNA sample. This convenient spin-column format eliminates the need for expensive resins or toxic organic compounds such as phenol and chloroform, thereby making it possible to process multiple samples in parallel. Purified DNA can be used in T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions.
Rapid – Purification of PCR products in less than 10 minutes
Safe – No Phenol/chloroform extractions
Versatile – Spin and vacuum formats available
High-quality – DNA is suitable for a variety of downstream applications
Downstream application: T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions
Elution volume: 30-50 µL
Starting materials: sDNA, dsDNA, PCR products
Starting amount: 5-50 µL
DNA recovered: >90% recovery, 100 bp to 10 kb
Processing mode: Manual (centrifugation or vacuum)
Throughput: 1-24
DNA binding technology: Silica mini spin column
Processing time: <10 minutes
Special Notes: For clean-up of DNA fragments < 200 bp, please follow the protocol modification outlined in the product manual
For more details, please follow https://uk.vwr.com/store/product?keyword=D6492-00
