The E.Z.N.A.® Mollusc DNA Kit is designed for efficient purification of genomic DNA from molluscs, arthropods, round worms, flatworms and other invertebrate tissues rich in mucopolysaccharides. Fresh and frozen samples that have been preserved in alcohol or DNE can be used with this kit. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind matrix. Samples are homogenized and chloroform is added to remove mucopolysaccharides. Following a rapid alcohol precipitation step, DNA is further purified and all salts, proteins and other contaminants are removed. High-quality genomic DNA is suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
Rapid – DNA isolation under 20 minutes following lysis
Reliable – Optimized buffer system guarantee pure DNA every time
High-quality – Purified DNA suitable for any application
Flexible – Optimized buffer system can isolate DNA from a wide range of samples
Starting Amount: 50 mg
Starting Material: Molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides