Product name: PCNA(1C11) Monoclonal Antibody
Applications: WB, IHC-P, IF(IHC-P)
Reactivity: Human, Mouse, Rat, Rabbit
Conjugation: Unconjugated
Host: Mouse
Source: KLH conjugated synthetic peptide derived from human PCNA
Immunogen range: 200-250/261
Clonality: Monoclonal
Clone: #1C11
Isotype: IgG
Concentration: 1ug/ul
Purification: Purified by Protein G.
Storage buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage condition: Store at -20°C for 12 months
TARGET
Gene ID: 5111
Swiss Prot: P12004
Subcellular location: Nucleus
Synonyms: ATLD2; Proliferating cell nuclear antigen; PCNA; Cyclin
Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
*Image title: Lane 1: NIH/3T3 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
For more details, please follow https://www.biossusa.com/products/bsm-2006m
