Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂
VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.
Ideal choice for routine applications
High performance, thermostable DNA polymerase
Optimal for TA cloning
Taq DNA polymerase concentration: 5 Units/μl
10X Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8,3; KCl, 15 mM MgCl₂, 1% Triton™ X-100
Application: Standard amplification
Size: 1 x 0.05 ml + 3 x 1.5 ml
Buffer composition: 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Amplicon size: ≤ 5kb
Units: 250
Exonuclease activity: 5’→3’
Product overhang: 3'A
Storage conditions: – 20 °C (long term), + 4 °C (6 months)
For more details, please follow https://uk.vwr.com/store/product?keyword=733-1300
